Author:
Segal WA, Pirnazar JR, Arens M, Pepose JS.
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Date Posted: Fri, October 12 2001, 12:01:43 PDT
Disinfection of Goldmann tonometers after contamination with hepatitis C virus.
Segal WA, Pirnazar JR, Arens M, Pepose JS.
Department of Ophthalmology and Visual Sciences, Washington University
School of Medicine, St Louis, Missouri, USA
PURPOSE: To compare methods of disinfecting Goldmann tonometer tips
inoculated with hepatitis C virus.
METHODS: Hepatitis C virus was placed on
Goldmann tonometer tips, air dried, and then disinfected by dry gauze
wipes, isopropyl alcohol wipes, cold water washes, povidone iodine 10%
wipes, and hydrogen peroxide or isopropyl alcohol soaks followed by a cold
water wash and dry. Hydrogen peroxide and isopropyl alcohol disinfection
techniques followed the Centers for Disease Control and Prevention
guidelines for prevention of possible transmission of human
immunodeficiency virus (HIV). After disinfection, samples from tonometer
tips were amplified by polymerase chain reaction to quantitate the amount
of hepatitis C virus RNA remaining.
RESULTS: Percentage of hepatitis C
virus RNA remaining after disinfection:
dry gauze wipes 95.65%,
isopropyl alcohol 5-second wipes 88.91%,
cold water wash 4.78%,
povidone iodine 10%
5-second wipes 0.72%,
hydrogen peroxide soak with cold water wash 0.07%,
isopropyl alcohol soak and cold water wash 0.02%.
CONCLUSIONS: After inoculation of Goldmann tonometer tips with hepatitis C virus, a 5-minute
soak in 3% hydrogen peroxide or 70% isopropyl alcohol followed by washing
in cold water resulted in the greatest reduction in hepatitis C virus RNA.
PMID: 11228293 [PubMed - indexed for MEDLINE]
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