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Date Posted: 19:13:56 05/27/00 Sat
Author: Pingu
Subject: Re: lac expression
In reply to: Armen Deuk 's message, "lac expression" on 17:50:49 07/27/99 Tue

> I was looking over Table 11-1 and I got pretty
> confused when I got to lanes 5 and 7. For lane 5, why
> is there any expression of the Z gene if it is mutated
> in the chromosome? I thought that the Z gene was
> cis-acting and the w.t. Z gene in the plasmid wouldn't
> affect it. The same question for lane 7.
> Thanx.

First, you have to make lane 5 triple in size, and put land 6 inside it. Then, splice them together, to create a new lane (call it lane 10), and dump your own damn genes in there.

Pingu

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