Tab. 11-1 -- Molecular Biology

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Date Posted: 23:59:12 07/28/99 Wed
Author: Devlin
Subject: Tab. 11-1

In my opinion the table 11-1 lines 5 and 7, it is cis acting. The way to tell is that the e.coli has a consitutive operator and the B.gal is mutant. So you would expect the B.gal to be - and -. Remember that a plasmid is not in the genome but it outside and can take the place of the e.coli genome incase of mutations as in the b.gal. If the b.gal gene was trans then instead of getting - and + you would get + and +. So for the b.gal gene, the plasmid takes over for it and it has a wt genotype thus lacking iptg gives it - but with iptg it is +, Then back on the e.coli chrom. the constitutive operater gives + and + expression for the lac permease gene and the plasmid is not needed because as far as the cell is concerned it has a functional copy of the gene. same as seven. just the the lac permease gene is mutated on the e.coli genome so it uses the wt plasmid for that sequence which again gives it the - and +. If this doesnt make since (one its late) then email me and I will try to re-explain it. DRAMBO95@hotmail.com.

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