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Date Posted: 03:39:44 05/26/04 Wed
Author: Dr. R.V. Vyas
Subject: Information for Biocontrol Scientists





Status of Paecilomyces lilacinus For Biological Control of Root-knot Nematodes IN Gujarat, INDIA

Dr. R.V. Vyas
Associate Professor (Invertebrate Pathologist)
Department of Nematology,B. A. College of Agriculture,
Anand Agricultural University, Anand - 388 110 India


New Bionematicide Effective Against Root-knot Nematodes

Biological control of root-knot disease (Meloidogyne spp.) by Paecilomyces lilacinus in groundnut (peanut) and cotton has been successfully achieved. Nematode egg parasitized by biocontrol fungus, P. lilacinus has provided fairly effective check of root-knot disease under field conditions. Three years pooled results indicated reduction of root-knot disease by 34.7 % in groundnut and 28.7 % in cotton enhancing 19. 1 % and 10.7 % crop yield, respectively over control when fungus was applied @ 25 kg/ha (spore dust/granules having 109 conidia/g based on rice grain substrate carrier).
Moreover in double whammy approach with nematicides and/or soil organic amendments, fungus gave better control of root-knot disease in cotton, chickpea, banana and tomato nursery over the last 8 years. A simple mass production technique of the fungus based on solid substrate fermentation techniques using broken rice grain waste and a new high-tech sodium alginate granular formulation technique have been developed for successful use of this bio-nematicide under field conditions.
Source:
1. Bionematicide information: GAU NEWS (3) : April 2000.
2. REVIEW: Vyas R. V. and Patel D. J. (2001) Biological control of root-knot nematodes in India. Annual Review of Plant Pathology (Vol.1) pp 137-154

Milestones:

Ø The entomopathogenic fungi viz. Beauveria brongniartii and Metarhizium anisopliae were proved to be parasitic on eggs of root-knot nematodes.

Ø A simple mass production technique of the biocontrol fungus, Paecilomyces lilacinus based on solid substrate fermentation technique using broken rice grain waste and a new high-tech sodium alginate granular formulation technique have been developed.

Ø Biological control of root-knot disease by nematode egg parasitizing fungus, Paecilomyces lilacinus in groundnut and cotton has been successfully demonstrated. Reduction of root-knot nematode by 34.7 % in groundnut and 28.7 % in cotton increasing 19. 1 % and 10.7 % crop yield, respectively over control was achieved when the fungus was applied at 25 kg/ha (spore dust/granules having 109 conidia/g based on rice grain substrate carrier).

Ø In double whammy approach, Paecilomyces lilacinus with nematicides and/or soil organic amendments gave better control of root-knot disease in cotton, chickpea, and banana and tomato nursery over last 8 years.

Ø Farmer’s field demonstration trials on P. lilacinus were carried out at village Telnar Dist. Kheda in cotton and Groundnut crops during Kharif 1999 followed by a farmers meeting on Sept.9, 1999 in which more than 200 farmers have participated.

Ø In vitro mass production technique for native entomophilic nematode, Steinernema sp. has been developed and standardized for the first time.

Ø Entomopathogenic nematodes proved to be effective for management of Helicoverpa armigera and Plutella xylostella under field conditions, Holotrichia consanguinea and Agrotis ipsilon in pot conditions on cotton, pigeonpea, chickpea, cress, groundnut and potato.*
· EPN information: GAU NEWS 4(1) October2001.

Conclusions of P.lilacinus studies come out as GAU Recommendations :

1. It is recommended that application of bio-nematicide, Paecilomyces lilacinus spore dust with substrate carrier @ 50 kg/ha (109 conidia/g product) was found effective for management of Meloidogyne javanica (patho type 2) , which was at par with carbofuran @ 1kg/ha(1998-99)

2. It is recommended that application of bio-nematicide, Paecilomyces lilacinus @ 25kg/ha (granules having spore load 5 x 108 conidia/g ) with phenamiphos @ 1kg/ha was found most effective for management of root-knot nematodes,Meloidogyne spp. with highest yield of cotton. Phenamiphos alone and P. lilacinus with Carbofuran @ 1kg/ha were next effective treatments. P. lilacinus alone was at par with carbofuran , the popular nematicide(1999-2000)

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